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A
single bottle aerobic respirometer for the laboratory measurement
of oxygen consumption rate and derived parameters in aerobic
cultures.
This
open system, allowing additions of test substances or nutrients
throughout the testing period, unlike those respirometers
based on pressure reduction techniques. It also supplies
air to the culture, hence never being oxygen limiting like
some simpler barometric systems.
The
measurement principal is that of DO concentration measurement
over time. Temperature stability is achieved with a self
contained peltier heating/cooling system.
Three modes of operation are available:
- Static
- this just measures a single oxygen decay of a pre-aerated
sample, giving the OUR or SOUR for that sample. Very useful
for rapid checks on ASP functionality.
- Cyclic
– This measures multiple oxygen decay curves between
fixed DO set points, allowing construction of a long term
respirogram.
-
Dynamic – This measures the variation
in respiration rate through the treatment of a sample.
Max respiration rates are determined as are total oxygen
demands. It is particularly useful for rapid comparative
tests,cumulative oxygen requirement tests, relative bioactivity
tests, Kinetic parameter determinations.
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